Informatics on High-throughput Sequencing Data (2016)

Course Objectives

A poster announcing this workshop can be found here

With the introduction of high-throughput sequencing platforms, it is becoming feasible to consider sequencing approaches to address many research projects. However, knowing how to manage and interpret the large volume of sequence data resulting from such technologies is less clear. The CBW has developed a popular 2-day course covering the bioinformatics tools available for managing and interpreting high-throughput sequencing data, where the focus is on Illumina reads although the information is applicable to all sequencer reads.

Beginning with an understanding of the workflow involved to move from platform images to sequence generation, participants will gain practical experience and skills to be able to:

  • Assess sequence quality
  • Map sequence data onto a reference genome (required)
  • Quantify sequence data
  • Integrate biological context with sequence information

Course Material

Open Access LogoCanadian Bioinformatics Workshops promotes open access. Past workshop content is available under a Creative Commons License.

Module 1 - Introduction to HT-seq and Cloud Computing (2016) (Faculty: Zhibin Lu)

application/pdf iconPDF (12MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPT (12MB)

Module 2 - Genome Alignment (2016) (Faculty: Mathieu Bourgey)

application/pdf iconPDF (3MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPT (5MB)

Module 3 - Genome Visualization (2016) (Faculty: Florence Cavalli)

application/pdf iconPDF (6MB) | application/vnd.ms-powerpoint iconPPT (6MB)

Module 4 - De Novo Assembly (2016) (Faculty: Jared Simpson)

application/pdf iconPDF (1MB)

Module 5 - Genome Variation (2016) (Faculty: Guillaume Bourque)

application/pdf iconPDF (3MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPT (2MB)

Module 6 - Genome Structural Variation (2016) (Faculty: Guillaume Bourque)

application/pdf iconPDF (6MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPT (6MB)

Module 7 - Bringing it Together with Galaxy (2016) (Faculty: David Morais)

application/pdf iconPDF (10MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPT (11MB)