Analysis of Metagenomic Data (2016)

Course Objectives

A poster announcing this workshop can be found here

Metagenomics, the sequencing of DNA directly from a sample without first culturing and isolating the organisms, has become the principal tool of “meta-omic” analysis. It can be used to explore the diversity, function, and ecology of microbial communities. The CBW has developed a 3-day course providing an introduction to metagenomic data analysis followed by hands-on practical tutorials demonstrating the use of metagenome analysis tools. The tutorials are designed as self-contained units that include example data and detailed instructions for installation of all required bioinformatics tools.

Participants will gain practical experience and skills to be able to:

  • Design appropriate microbiome-focused experiments
  • Understand the advantages and limitations of metagenomic data analysis
  • Devise an appropriate bioinformatics workflow for processing and analyzing metagenomic sequence data (marker-gene, shotgun metagenomic, and metatranscriptomic data)
  • Apply appropriate statistics to undertake rigourous data analysis
  • Visualize datasets to gain intuitive insights into the composition and/or activity of their data set

Course Material

Open Access LogoCanadian Bioinformatics Workshops promotes open access. Past workshop content is available under a Creative Commons License.

Module 1 - Introduction to Metagenomics (2016) (Faculty: William Hsiao)

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Module 2 - Marker Gene-based Analysis of Taxonomic Composition (2016) (Faculty: William Hsiao)

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Module 3 - PICRUSt (Faculty: Morgan Langille)

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Module 4 - Metagenomic Taxonomic Composition (2016) (Faculty: Morgan Langille)

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Module 5 - Metagenomic Functional Composition (2016) (Faculty: Morgan Langille)

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Module 6 - Metatranscriptomics (2016) (Faculty: John Parkinson)

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Module 7 - Biomarker Selection (2016) (Faculty: Fiona Brinkman)

application/pdf iconPDF (7MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPT (6MB) |  iconYouTube (0KB)