Informatics on High Throughput Sequencing Data (2013)

Course Objectives

With the introduction of high-throughput sequencing platforms from Illumina, Roche and ABI, it is becoming feasible to consider sequencing approaches to address many research projects. However, knowing how to manage and interpret the large volume of sequence data resulting from such technologies is less clear. The CBW has developed a 2-day course covering the bioinformatics tools available for managing and interpreting high-throughput sequencing data.

Beginning with an understanding of the workflow involved to move from platform images to sequence generation, participants will gain practical experience and skills to be able to:

  • Assess sequence quality
  • Map sequence data onto a reference genome
  • Quantify sequence data
  • Integrate biological context with sequence information

Course Material

Open Access LogoCanadian Bioinformatics Workshops promotes open access. Past workshop content is available under a Creative Commons License.

Module 1 - Reference-guided Genome Alignment (2013) (Faculty: Michael Stromberg)

application/pdf iconPDF (5MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPTX (3MB) | video/mp4 iconMP4 (37MB)

Module 2 - Small-variant calling and annotation (2013) (Faculty: Michael Stromberg)

application/pdf iconPDF (14MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPTX (11MB) | video/mp4 iconMP4 (28MB)

Module 3 - Structural variant calling (includes CNVs) (2013) (Faculty: Aaron Quinlan)

application/pdf iconPDF (16MB) | video/mp4 iconMP4 (404MB)

Module 4 - Visual Analysis (2013) (Faculty: Marc Fiume)

application/pdf iconPDF (3MB) | application/vnd.ms-powerpoint iconPPT (4MB) | video/mp4 iconMP4 (75MB)

Module 5 - Bringing it all together: Galaxy (2013) (Faculty: Francis Ouellette)

application/pdf iconPDF (12MB) | application/vnd.openxmlformats-officedocument.presentationml.presentation iconPPT (10MB) | video/mp4 iconMP4 (360MB)