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CHOPCHOP is a web tool for selecting the optimum target sites for CRISPR/Cas9- or TALEN-directed mutagenesis. It can be run with three basic input options, or with additional advanced parameters. The basic input comprises: (i) a gene name, genomic coordinates or a pasted sequence (accepting RefSeq, ENSEMBL, FlyBase and WormBase gene IDs); (ii) a growing list of organisms (including human, mouse, zebrafish, etc.); and (iii) the choice between CRISPR/Cas9 or TALEN mode.
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